Construct: shRNA TRCN0000093818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144908.1-526s1c1
DNA Barcode:
GCACGCCTTTAATATGCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt11 (231050)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 11
Transcript:
RefSeq NM_144908.1 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGCCTTTAATATGCTCAT
Hairpin Sequence:
5'-CCGG-GCACGCCTTTAATATGCTCAT-CTCGAG-ATGAGCATATTAAAGGCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGCCTTTAATATGCTCATCTCGAGATGAGCATATTAAAGGCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGCCTTTAATATGCTCATCTCGAGATGAGCATATTAAAGGCGTGC-3'

Other clones with same target sequence:

(none)