Construct: shRNA TRCN0000093835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358849.1-351s1c1
DNA Barcode:
GCAGAATATAAGAAAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qtrt2 (106248)
Gene Description:
queuine tRNA-ribosyltransferase accessory subunit 2
Transcript:
RefSeq XM_358849.1 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATATAAGAAAGGAGTT
Hairpin Sequence:
5'-CCGG-GCAGAATATAAGAAAGGAGTT-CTCGAG-AACTCCTTTCTTATATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATATAAGAAAGGAGTTCTCGAGAACTCCTTTCTTATATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATATAAGAAAGGAGTTCTCGAGAACTCCTTTCTTATATTCTGC-3'

Other clones with same target sequence:

(none)