Construct: shRNA TRCN0000093899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145218.2-3005s1c1
DNA Barcode:
CGCTTACTGCTCAGCTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wbscr17 (212996)
Gene Description:
Williams-Beuren syndrome chromosome region 17 homolog (human)
Transcript:
RefSeq NM_145218.2 (NON-CURRENT)
Match location:
Position 3005 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTACTGCTCAGCTCATTT
Hairpin Sequence:
5'-CCGG-CGCTTACTGCTCAGCTCATTT-CTCGAG-AAATGAGCTGAGCAGTAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTACTGCTCAGCTCATTTCTCGAGAAATGAGCTGAGCAGTAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTACTGCTCAGCTCATTTCTCGAGAAATGAGCTGAGCAGTAAGCG-3'

Other clones with same target sequence:

(none)