Construct: shRNA TRCN0000093909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152811.1-1724s1c1
DNA Barcode:
GCTCTCATAGATGCACTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt2b1 (71773)
Gene Description:
UDP glucuronosyltransferase 2 family, polypeptide B1
Transcript:
RefSeq NM_152811.1 (CURRENT)
Match location:
Position 1724 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCATAGATGCACTATAT
Hairpin Sequence:
5'-CCGG-GCTCTCATAGATGCACTATAT-CTCGAG-ATATAGTGCATCTATGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCATAGATGCACTATATCTCGAGATATAGTGCATCTATGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCATAGATGCACTATATCTCGAGATATAGTGCATCTATGAGAGC-3'

Other clones with same target sequence:

(none)