Construct: shRNA TRCN0000093915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010687.1-2175s1c1
DNA Barcode:
CCTGAGTATGATCGGAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Large1 (16795)
Gene Description:
LARGE xylosyl- and glucuronyltransferase 1
Transcript:
RefSeq NM_010687.1 (NON-CURRENT)
Match location:
Position 2175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTATGATCGGAGATTT
Hairpin Sequence:
5'-CCGG-CCTGAGTATGATCGGAGATTT-CTCGAG-AAATCTCCGATCATACTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTATGATCGGAGATTTCTCGAGAAATCTCCGATCATACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTATGATCGGAGATTTCTCGAGAAATCTCCGATCATACTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000312358