Construct: shRNA TRCN0000093990

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127943.2-402s1c1
DNA Barcode:
CATCGACTTATAGACGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt3a1 (105887)
Gene Description:
UDP glycosyltransferases 3 family, polypeptide A1
Transcript:
RefSeq XM_127943.2 (NON-CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093990)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGACTTATAGACGAATAT
Hairpin Sequence:
5'-CCGG-CATCGACTTATAGACGAATAT-CTCGAG-ATATTCGTCTATAAGTCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGACTTATAGACGAATATCTCGAGATATTCGTCTATAAGTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGACTTATAGACGAATATCTCGAGATATTCGTCTATAAGTCGATG-3'

Other clones with same target sequence:

(none)