Construct: shRNA TRCN0000093998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009182.2-844s1c1
DNA Barcode:
GCAACTGTAACGAGAACGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St8sia3 (20451)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Transcript:
RefSeq NM_009182.2 (NON-CURRENT)
Match location:
Position 844 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTGTAACGAGAACGCTA
Hairpin Sequence:
5'-CCGG-GCAACTGTAACGAGAACGCTA-CTCGAG-TAGCGTTCTCGTTACAGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTGTAACGAGAACGCTACTCGAGTAGCGTTCTCGTTACAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTGTAACGAGAACGCTACTCGAGTAGCGTTCTCGTTACAGTTGC-3'

Other clones with same target sequence:

(none)