Construct: shRNA TRCN0000094061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019548.2-1746s1c1
DNA Barcode:
CGAAAGTCTAAGCATCCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tro (56191)
Gene Description:
trophinin
Transcript:
RefSeq NM_019548.2 (NON-CURRENT)
Match location:
Position 1746 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGTCTAAGCATCCCAAT
Hairpin Sequence:
5'-CCGG-CGAAAGTCTAAGCATCCCAAT-CTCGAG-ATTGGGATGCTTAGACTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGTCTAAGCATCCCAATCTCGAGATTGGGATGCTTAGACTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGTCTAAGCATCCCAATCTCGAGATTGGGATGCTTAGACTTTCG-3'

Other clones with same target sequence:

(none)