Construct: shRNA TRCN0000094072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013504.2-2301s1c1
DNA Barcode:
CCAGATGATGTAGCTCAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dsc1 (13505)
Gene Description:
desmocollin 1
Transcript:
RefSeq NM_013504.2 (NON-CURRENT)
Match location:
Position 2301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGATGTAGCTCAGCAA
Hairpin Sequence:
5'-CCGG-CCAGATGATGTAGCTCAGCAA-CTCGAG-TTGCTGAGCTACATCATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGATGTAGCTCAGCAACTCGAGTTGCTGAGCTACATCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGATGTAGCTCAGCAACTCGAGTTGCTGAGCTACATCATCTGG-3'

Other clones with same target sequence:

(none)