Construct: shRNA TRCN0000094133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178748.3-1411s1c1
DNA Barcode:
CGTAACCTTTGAGCCTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Egflam (268780)
Gene Description:
EGF-like, fibronectin type III and laminin G domains
Transcript:
RefSeq NM_178748.3 (NON-CURRENT)
Match location:
Position 1411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAACCTTTGAGCCTCTGAA
Hairpin Sequence:
5'-CCGG-CGTAACCTTTGAGCCTCTGAA-CTCGAG-TTCAGAGGCTCAAAGGTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAACCTTTGAGCCTCTGAACTCGAGTTCAGAGGCTCAAAGGTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAACCTTTGAGCCTCTGAACTCGAGTTCAGAGGCTCAAAGGTTACG-3'

Other clones with same target sequence:

(none)