Construct: shRNA TRCN0000094141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011693.2-927s1c1
DNA Barcode:
CCAAATTGATTCTACACTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vcam1 (22329)
Gene Description:
vascular cell adhesion molecule 1
Transcript:
RefSeq NM_011693.2 (NON-CURRENT)
Match location:
Position 927 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATTGATTCTACACTCAA
Hairpin Sequence:
5'-CCGG-CCAAATTGATTCTACACTCAA-CTCGAG-TTGAGTGTAGAATCAATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATTGATTCTACACTCAACTCGAGTTGAGTGTAGAATCAATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATTGATTCTACACTCAACTCGAGTTGAGTGTAGAATCAATTTGG-3'

Other clones with same target sequence:

(none)