Construct: shRNA TRCN0000094163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025711.2-238s1c1
DNA Barcode:
GCTCTGCCAAACCCTTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aspn (66695)
Gene Description:
asporin
Transcript:
RefSeq NM_025711.2 (NON-CURRENT)
Match location:
Position 238 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGCCAAACCCTTCTTTA
Hairpin Sequence:
5'-CCGG-GCTCTGCCAAACCCTTCTTTA-CTCGAG-TAAAGAAGGGTTTGGCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGCCAAACCCTTCTTTACTCGAGTAAAGAAGGGTTTGGCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGCCAAACCCTTCTTTACTCGAGTAAAGAAGGGTTTGGCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000352111