Construct: shRNA TRCN0000094200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033576.1-1239s1c1
DNA Barcode:
CCCGGAATACAACATTACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhgb4 (93701)
Gene Description:
protocadherin gamma subfamily B, 4
Transcript:
RefSeq NM_033576.1 (CURRENT)
Match location:
Position 1239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGAATACAACATTACTAT
Hairpin Sequence:
5'-CCGG-CCCGGAATACAACATTACTAT-CTCGAG-ATAGTAATGTTGTATTCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGAATACAACATTACTATCTCGAGATAGTAATGTTGTATTCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGAATACAACATTACTATCTCGAGATAGTAATGTTGTATTCCGGG-3'

Other clones with same target sequence:

(none)