Construct: shRNA TRCN0000094243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033593.2-2324s1c1
DNA Barcode:
GCAGTCTTCGAGAGATGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhga10 (93722)
Gene Description:
protocadherin gamma subfamily A, 10
Transcript:
RefSeq NM_033593.2 (NON-CURRENT)
Match location:
Position 2324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCTTCGAGAGATGGATT
Hairpin Sequence:
5'-CCGG-GCAGTCTTCGAGAGATGGATT-CTCGAG-AATCCATCTCTCGAAGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCTTCGAGAGATGGATTCTCGAGAATCCATCTCTCGAAGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCTTCGAGAGATGGATTCTCGAGAATCCATCTCTCGAAGACTGC-3'

Other clones with same target sequence:

(none)