Construct: shRNA TRCN0000094341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019459.1-675s1c1
DNA Barcode:
CACTCCCATAAAGGCTTCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nphs1 (54631)
Gene Description:
nephrosis 1, nephrin
Transcript:
RefSeq NM_019459.1 (NON-CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCCCATAAAGGCTTCGTT
Hairpin Sequence:
5'-CCGG-CACTCCCATAAAGGCTTCGTT-CTCGAG-AACGAAGCCTTTATGGGAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCCCATAAAGGCTTCGTTCTCGAGAACGAAGCCTTTATGGGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCCCATAAAGGCTTCGTTCTCGAGAACGAAGCCTTTATGGGAGTG-3'

Other clones with same target sequence:

(none)