Construct: shRNA TRCN0000094354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007932.1-2589s1c1
DNA Barcode:
CCATGCTGCTTAGAAGCCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eng (13805)
Gene Description:
endoglin
Transcript:
RefSeq NM_007932.1 (NON-CURRENT)
Match location:
Position 2589 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTGCTTAGAAGCCTAA
Hairpin Sequence:
5'-CCGG-CCATGCTGCTTAGAAGCCTAA-CTCGAG-TTAGGCTTCTAAGCAGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTGCTTAGAAGCCTAACTCGAGTTAGGCTTCTAAGCAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTGCTTAGAAGCCTAACTCGAGTTAGGCTTCTAAGCAGCATGG-3'

Other clones with same target sequence:

(none)