Construct: shRNA TRCN0000094461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054077.2-870s1c1
DNA Barcode:
CTACCTGGATAGCAACAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prelp (116847)
Gene Description:
proline arginine-rich end leucine-rich repeat
Transcript:
RefSeq NM_054077.2 (NON-CURRENT)
Match location:
Position 870 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCTGGATAGCAACAAGAT
Hairpin Sequence:
5'-CCGG-CTACCTGGATAGCAACAAGAT-CTCGAG-ATCTTGTTGCTATCCAGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCTGGATAGCAACAAGATCTCGAGATCTTGTTGCTATCCAGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCTGGATAGCAACAAGATCTCGAGATCTTGTTGCTATCCAGGTAG-3'

Other clones with same target sequence:

  1. TRCN0000318135