Construct: shRNA TRCN0000094497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053137.1-1644s1c1
DNA Barcode:
CAATTCACCTTTCGTGCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhb12 (93883)
Gene Description:
protocadherin beta 12
Transcript:
RefSeq NM_053137.1 (NON-CURRENT)
Match location:
Position 1644 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTCACCTTTCGTGCTCTA
Hairpin Sequence:
5'-CCGG-CAATTCACCTTTCGTGCTCTA-CTCGAG-TAGAGCACGAAAGGTGAATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTCACCTTTCGTGCTCTACTCGAGTAGAGCACGAAAGGTGAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTCACCTTTCGTGCTCTACTCGAGTAGAGCACGAAAGGTGAATTG-3'

Other clones with same target sequence:

(none)