Construct: shRNA TRCN0000094514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030699.1-3272s1c1
DNA Barcode:
CCAGACAATCTGTTAATGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntng1 (80883)
Gene Description:
netrin G1
Transcript:
RefSeq NM_030699.1 (NON-CURRENT)
Match location:
Position 3272 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACAATCTGTTAATGTAT
Hairpin Sequence:
5'-CCGG-CCAGACAATCTGTTAATGTAT-CTCGAG-ATACATTAACAGATTGTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACAATCTGTTAATGTATCTCGAGATACATTAACAGATTGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACAATCTGTTAATGTATCTCGAGATACATTAACAGATTGTCTGG-3'

Other clones with same target sequence:

(none)