Construct: shRNA TRCN0000094552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008478.2-3142s1c1
DNA Barcode:
CATATCTTGTTCAAAGCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
L1cam (16728)
Gene Description:
L1 cell adhesion molecule
Transcript:
RefSeq NM_008478.2 (NON-CURRENT)
Match location:
Position 3142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATATCTTGTTCAAAGCCTTA
Hairpin Sequence:
5'-CCGG-CATATCTTGTTCAAAGCCTTA-CTCGAG-TAAGGCTTTGAACAAGATATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATATCTTGTTCAAAGCCTTACTCGAGTAAGGCTTTGAACAAGATATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATATCTTGTTCAAAGCCTTACTCGAGTAAGGCTTTGAACAAGATATG-3'

Other clones with same target sequence:

(none)