Construct: shRNA TRCN0000094582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130867.2-1897s1c1
DNA Barcode:
CCCACCAATGGTTATTACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kirrel (170643)
Gene Description:
kin of IRRE like (Drosophila)
Transcript:
RefSeq NM_130867.2 (NON-CURRENT)
Match location:
Position 1897 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACCAATGGTTATTACAAT
Hairpin Sequence:
5'-CCGG-CCCACCAATGGTTATTACAAT-CTCGAG-ATTGTAATAACCATTGGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACCAATGGTTATTACAATCTCGAGATTGTAATAACCATTGGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACCAATGGTTATTACAATCTCGAGATTGTAATAACCATTGGTGGG-3'

Other clones with same target sequence:

(none)