Construct: shRNA TRCN0000094589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172694.1-2050s1c1
DNA Barcode:
GCCTGTCTGTTGCTCTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Megf9 (230316)
Gene Description:
multiple EGF-like-domains 9
Transcript:
RefSeq NM_172694.1 (NON-CURRENT)
Match location:
Position 2050 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTCTGTTGCTCTTAATT
Hairpin Sequence:
5'-CCGG-GCCTGTCTGTTGCTCTTAATT-CTCGAG-AATTAAGAGCAACAGACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTCTGTTGCTCTTAATTCTCGAGAATTAAGAGCAACAGACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTCTGTTGCTCTTAATTCTCGAGAATTAAGAGCAACAGACAGGC-3'

Other clones with same target sequence:

(none)