Construct: shRNA TRCN0000094737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022319.2-1956s1c1
DNA Barcode:
CTATATCAACTCCCGGCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clstn2 (64085)
Gene Description:
calsyntenin 2
Transcript:
RefSeq NM_022319.2 (CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATATCAACTCCCGGCAATT
Hairpin Sequence:
5'-CCGG-CTATATCAACTCCCGGCAATT-CTCGAG-AATTGCCGGGAGTTGATATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATATCAACTCCCGGCAATTCTCGAGAATTGCCGGGAGTTGATATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATATCAACTCCCGGCAATTCTCGAGAATTGCCGGGAGTTGATATAG-3'

Other clones with same target sequence:

(none)