Construct: shRNA TRCN0000094753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033583.2-657s1c1
DNA Barcode:
CCACTTCTCTCTGCATGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhgc5 (93708)
Gene Description:
protocadherin gamma subfamily C, 5
Transcript:
RefSeq NM_033583.2 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCTCTCTGCATGTGAA
Hairpin Sequence:
5'-CCGG-CCACTTCTCTCTGCATGTGAA-CTCGAG-TTCACATGCAGAGAGAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCTCTCTGCATGTGAACTCGAGTTCACATGCAGAGAGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCTCTCTGCATGTGAACTCGAGTTCACATGCAGAGAGAAGTGG-3'

Other clones with same target sequence:

(none)