Construct: shRNA TRCN0000094793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009154.1-2573s1c1
DNA Barcode:
CCTGGTCTAAATGTTCAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema5a (20356)
Gene Description:
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Transcript:
RefSeq NM_009154.1 (NON-CURRENT)
Match location:
Position 2573 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCTAAATGTTCAGCAA
Hairpin Sequence:
5'-CCGG-CCTGGTCTAAATGTTCAGCAA-CTCGAG-TTGCTGAACATTTAGACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCTAAATGTTCAGCAACTCGAGTTGCTGAACATTTAGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCTAAATGTTCAGCAACTCGAGTTGCTGAACATTTAGACCAGG-3'

Other clones with same target sequence:

(none)