Construct: shRNA TRCN0000094827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021320.2-475s1c1
DNA Barcode:
GCCCAAGTGTGATAAATGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntn4 (57764)
Gene Description:
netrin 4
Transcript:
RefSeq NM_021320.2 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAGTGTGATAAATGCAA
Hairpin Sequence:
5'-CCGG-GCCCAAGTGTGATAAATGCAA-CTCGAG-TTGCATTTATCACACTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAGTGTGATAAATGCAACTCGAGTTGCATTTATCACACTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAGTGTGATAAATGCAACTCGAGTTGCATTTATCACACTTGGGC-3'

Other clones with same target sequence:

(none)