Construct: shRNA TRCN0000094831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009866.3-1853s1c1
DNA Barcode:
GCAGAAATTCACAACAGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdh11 (12552)
Gene Description:
cadherin 11
Transcript:
RefSeq NM_009866.3 (NON-CURRENT)
Match location:
Position 1853 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATTCACAACAGACAT
Hairpin Sequence:
5'-CCGG-GCAGAAATTCACAACAGACAT-CTCGAG-ATGTCTGTTGTGAATTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATTCACAACAGACATCTCGAGATGTCTGTTGTGAATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATTCACAACAGACATCTCGAGATGTCTGTTGTGAATTTCTGC-3'

Other clones with same target sequence:

(none)