Construct: shRNA TRCN0000094840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033594.2-1785s1c1
DNA Barcode:
CCCACCATTAAGCAGCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhga11 (93723)
Gene Description:
protocadherin gamma subfamily A, 11
Transcript:
RefSeq NM_033594.2 (CURRENT)
Match location:
Position 1785 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACCATTAAGCAGCAATAT
Hairpin Sequence:
5'-CCGG-CCCACCATTAAGCAGCAATAT-CTCGAG-ATATTGCTGCTTAATGGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACCATTAAGCAGCAATATCTCGAGATATTGCTGCTTAATGGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACCATTAAGCAGCAATATCTCGAGATATTGCTGCTTAATGGTGGG-3'

Other clones with same target sequence:

(none)