Construct: shRNA TRCN0000094877

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201243.1-2645s1c1
DNA Barcode:
ACAAATTCATTATCCCAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdha8 (353235)
Gene Description:
protocadherin alpha 8
Transcript:
RefSeq NM_201243.1 (CURRENT)
Match location:
Position 2645 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094877)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAATTCATTATCCCAGGAT
Hairpin Sequence:
5'-CCGG-ACAAATTCATTATCCCAGGAT-CTCGAG-ATCCTGGGATAATGAATTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAATTCATTATCCCAGGATCTCGAGATCCTGGGATAATGAATTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAATTCATTATCCCAGGATCTCGAGATCCTGGGATAATGAATTTGT-3'

Other clones with same target sequence:

(none)