Construct: shRNA TRCN0000095003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029988.1-4771s1c1
DNA Barcode:
CCTCGTTATGAGGCAAATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fat2 (245827)
Gene Description:
FAT atypical cadherin 2
Transcript:
RefSeq NM_001029988.1 (NON-CURRENT)
Match location:
Position 4771 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTTATGAGGCAAATGTT
Hairpin Sequence:
5'-CCGG-CCTCGTTATGAGGCAAATGTT-CTCGAG-AACATTTGCCTCATAACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTTATGAGGCAAATGTTCTCGAGAACATTTGCCTCATAACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTTATGAGGCAAATGTTCTCGAGAACATTTGCCTCATAACGAGG-3'

Other clones with same target sequence:

(none)