Construct: shRNA TRCN0000095027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033589.1-1235s1c1
DNA Barcode:
GCGAACAGGTATCTTCGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhga6 (93714)
Gene Description:
protocadherin gamma subfamily A, 6
Transcript:
RefSeq NM_033589.1 (CURRENT)
Match location:
Position 1235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAACAGGTATCTTCGTATA
Hairpin Sequence:
5'-CCGG-GCGAACAGGTATCTTCGTATA-CTCGAG-TATACGAAGATACCTGTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAACAGGTATCTTCGTATACTCGAGTATACGAAGATACCTGTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAACAGGTATCTTCGTATACTCGAGTATACGAAGATACCTGTTCGC-3'

Other clones with same target sequence:

(none)