Construct: shRNA TRCN0000095108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172714.1-1346s1c1
DNA Barcode:
GAAGCCTTCAAGCCAAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lin54 (231506)
Gene Description:
lin-54 homolog (C. elegans)
Transcript:
RefSeq NM_172714.1 (NON-CURRENT)
Match location:
Position 1346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCCTTCAAGCCAAAGATA
Hairpin Sequence:
5'-CCGG-GAAGCCTTCAAGCCAAAGATA-CTCGAG-TATCTTTGGCTTGAAGGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCCTTCAAGCCAAAGATACTCGAGTATCTTTGGCTTGAAGGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCCTTCAAGCCAAAGATACTCGAGTATCTTTGGCTTGAAGGCTTC-3'

Other clones with same target sequence:

(none)