Construct: shRNA TRCN0000095113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007472.1-756s1c1
DNA Barcode:
GAAGATGCTGAGTTGGGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Noto (384452)
Gene Description:
notochord homeobox
Transcript:
RefSeq NM_001007472.1 (NON-CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGCTGAGTTGGGAATT
Hairpin Sequence:
5'-CCGG-GAAGATGCTGAGTTGGGAATT-CTCGAG-AATTCCCAACTCAGCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGCTGAGTTGGGAATTCTCGAGAATTCCCAACTCAGCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGCTGAGTTGGGAATTCTCGAGAATTCCCAACTCAGCATCTTC-3'

Other clones with same target sequence:

(none)