Construct: shRNA TRCN0000095132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153780.1-206s1c1
DNA Barcode:
CTACCAGAATCTCACTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2610044O15Rik8 (72139)
Gene Description:
RIKEN cDNA 2610044O15 gene
Transcript:
RefSeq NM_153780.1 (NON-CURRENT)
Match location:
Position 206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCAGAATCTCACTGATAT
Hairpin Sequence:
5'-CCGG-CTACCAGAATCTCACTGATAT-CTCGAG-ATATCAGTGAGATTCTGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCAGAATCTCACTGATATCTCGAGATATCAGTGAGATTCTGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCAGAATCTCACTGATATCTCGAGATATCAGTGAGATTCTGGTAG-3'

Other clones with same target sequence:

(none)