Construct: shRNA TRCN0000095204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024189.4-923s1c1
DNA Barcode:
GCAGTGTTATTGGTAAGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Yaf2 (67057)
Gene Description:
YY1 associated factor 2
Transcript:
RefSeq NM_024189.4 (NON-CURRENT)
Match location:
Position 923 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGTTATTGGTAAGTCTA
Hairpin Sequence:
5'-CCGG-GCAGTGTTATTGGTAAGTCTA-CTCGAG-TAGACTTACCAATAACACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGTTATTGGTAAGTCTACTCGAGTAGACTTACCAATAACACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGTTATTGGTAAGTCTACTCGAGTAGACTTACCAATAACACTGC-3'

Other clones with same target sequence:

  1. TRCN0000332379