Construct: shRNA TRCN0000095239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005867.1-3245s1c1
DNA Barcode:
CCAGCACTGATCCTTCTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmiz2 (52915)
Gene Description:
zinc finger, MIZ-type containing 2
Transcript:
RefSeq NM_001005867.1 (CURRENT)
Match location:
Position 3245 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCACTGATCCTTCTGTTT
Hairpin Sequence:
5'-CCGG-CCAGCACTGATCCTTCTGTTT-CTCGAG-AAACAGAAGGATCAGTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCACTGATCCTTCTGTTTCTCGAGAAACAGAAGGATCAGTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCACTGATCCTTCTGTTTCTCGAGAAACAGAAGGATCAGTGCTGG-3'

Other clones with same target sequence:

(none)