Construct: shRNA TRCN0000095267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017426.1-2519s1c1
DNA Barcode:
GCTGGATGAATCCATTCGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kdm6b (216850)
Gene Description:
KDM1 lysine (K)-specific demethylase 6B
Transcript:
RefSeq NM_001017426.1 (CURRENT)
Match location:
Position 2519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATGAATCCATTCGGAA
Hairpin Sequence:
5'-CCGG-GCTGGATGAATCCATTCGGAA-CTCGAG-TTCCGAATGGATTCATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATGAATCCATTCGGAACTCGAGTTCCGAATGGATTCATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATGAATCCATTCGGAACTCGAGTTCCGAATGGATTCATCCAGC-3'

Other clones with same target sequence:

(none)