Construct: shRNA TRCN0000095286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008601.1-847s1c1
DNA Barcode:
CGGTGGAACAAGGGAACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mitf (17342)
Gene Description:
microphthalmia-associated transcription factor
Transcript:
RefSeq NM_008601.1 (NON-CURRENT)
Match location:
Position 847 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGAACAAGGGAACCATT
Hairpin Sequence:
5'-CCGG-CGGTGGAACAAGGGAACCATT-CTCGAG-AATGGTTCCCTTGTTCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGAACAAGGGAACCATTCTCGAGAATGGTTCCCTTGTTCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGAACAAGGGAACCATTCTCGAGAATGGTTCCCTTGTTCCACCG-3'

Other clones with same target sequence:

(none)