Construct: shRNA TRCN0000095384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011274.2-2186s1c1
DNA Barcode:
CCTGGTTTAGATTGTGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uri1 (19777)
Gene Description:
URI1, prefoldin-like chaperone
Transcript:
RefSeq NM_011274.2 (NON-CURRENT)
Match location:
Position 2186 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTTTAGATTGTGTATAA
Hairpin Sequence:
5'-CCGG-CCTGGTTTAGATTGTGTATAA-CTCGAG-TTATACACAATCTAAACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTTTAGATTGTGTATAACTCGAGTTATACACAATCTAAACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTTTAGATTGTGTATAACTCGAGTTATACACAATCTAAACCAGG-3'

Other clones with same target sequence:

(none)