Construct: shRNA TRCN0000095454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009332.1-1788s1c1
DNA Barcode:
CGGGACTCCAAGTAGTAATGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf7l1 (21415)
Gene Description:
transcription factor 7 like 1 (T cell specific, HMG box)
Transcript:
RefSeq NM_009332.1 (NON-CURRENT)
Match location:
Position 1788 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACTCCAAGTAGTAATGA
Hairpin Sequence:
5'-CCGG-CGGGACTCCAAGTAGTAATGA-CTCGAG-TCATTACTACTTGGAGTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACTCCAAGTAGTAATGACTCGAGTCATTACTACTTGGAGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACTCCAAGTAGTAATGACTCGAGTCATTACTACTTGGAGTCCCG-3'

Other clones with same target sequence:

(none)