Construct: shRNA TRCN0000095540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153398.2-2270s1c1
DNA Barcode:
CCATCATCTTACAGATCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb24 (268294)
Gene Description:
zinc finger and BTB domain containing 24
Transcript:
RefSeq NM_153398.2 (NON-CURRENT)
Match location:
Position 2270 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCATCTTACAGATCCTTA
Hairpin Sequence:
5'-CCGG-CCATCATCTTACAGATCCTTA-CTCGAG-TAAGGATCTGTAAGATGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCATCTTACAGATCCTTACTCGAGTAAGGATCTGTAAGATGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCATCTTACAGATCCTTACTCGAGTAAGGATCTGTAAGATGATGG-3'

Other clones with same target sequence:

(none)