Construct: shRNA TRCN0000095610

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029999.3-301s1c1
DNA Barcode:
GCTCCATCCATGGAAGAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lbh (77889)
Gene Description:
limb-bud and heart
Transcript:
RefSeq NM_029999.3 (NON-CURRENT)
Match location:
Position 301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095610)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATCCATGGAAGAGATT
Hairpin Sequence:
5'-CCGG-GCTCCATCCATGGAAGAGATT-CTCGAG-AATCTCTTCCATGGATGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATCCATGGAAGAGATTCTCGAGAATCTCTTCCATGGATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATCCATGGAAGAGATTCTCGAGAATCTCTTCCATGGATGGAGC-3'

Other clones with same target sequence:

(none)