Construct: shRNA TRCN0000095666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019768.3-911s1c1
DNA Barcode:
CGCAGGGAAATGTTGATAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Morf4l2 (56397)
Gene Description:
mortality factor 4 like 2
Transcript:
RefSeq NM_019768.3 (NON-CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGGGAAATGTTGATAATA
Hairpin Sequence:
5'-CCGG-CGCAGGGAAATGTTGATAATA-CTCGAG-TATTATCAACATTTCCCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGGGAAATGTTGATAATACTCGAGTATTATCAACATTTCCCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGGGAAATGTTGATAATACTCGAGTATTATCAACATTTCCCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000332779