Construct: shRNA TRCN0000095679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146175.2-3043s1c1
DNA Barcode:
CCCAACCTGTATTTCTCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp282 (101095)
Gene Description:
zinc finger protein 282
Transcript:
RefSeq NM_146175.2 (NON-CURRENT)
Match location:
Position 3043 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACCTGTATTTCTCAATT
Hairpin Sequence:
5'-CCGG-CCCAACCTGTATTTCTCAATT-CTCGAG-AATTGAGAAATACAGGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACCTGTATTTCTCAATTCTCGAGAATTGAGAAATACAGGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACCTGTATTTCTCAATTCTCGAGAATTGAGAAATACAGGTTGGG-3'

Other clones with same target sequence:

(none)