Construct: shRNA TRCN0000095787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007958.1-1793s1c1
DNA Barcode:
CCTCCCTTCTAAACCAAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarcad1 (13990)
Gene Description:
SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1
Transcript:
RefSeq NM_007958.1 (CURRENT)
Match location:
Position 1793 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTTCTAAACCAAAGTT
Hairpin Sequence:
5'-CCGG-CCTCCCTTCTAAACCAAAGTT-CTCGAG-AACTTTGGTTTAGAAGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTTCTAAACCAAAGTTCTCGAGAACTTTGGTTTAGAAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTTCTAAACCAAAGTTCTCGAGAACTTTGGTTTAGAAGGGAGG-3'

Other clones with same target sequence:

(none)