Construct: shRNA TRCN0000095854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146176.1-2662s1c1
DNA Barcode:
CCCAGGAAGCAGGGAAGGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnot3 (232791)
Gene Description:
CCR4-NOT transcription complex, subunit 3
Transcript:
RefSeq NM_146176.1 (NON-CURRENT)
Match location:
Position 2662 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAAGCAGGGAAGGGAT
Hairpin Sequence:
5'-CCGG-CCCAGGAAGCAGGGAAGGGAT-CTCGAG-ATCCCTTCCCTGCTTCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAAGCAGGGAAGGGATCTCGAGATCCCTTCCCTGCTTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAAGCAGGGAAGGGATCTCGAGATCCCTTCCCTGCTTCCTGGG-3'

Other clones with same target sequence:

(none)