Construct: shRNA TRCN0000095905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009564.1-381s1c1
DNA Barcode:
GCTCCAGAGTTCGTCTTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp64 (22722)
Gene Description:
zinc finger protein 64
Transcript:
RefSeq NM_009564.1 (NON-CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCAGAGTTCGTCTTTGAA
Hairpin Sequence:
5'-CCGG-GCTCCAGAGTTCGTCTTTGAA-CTCGAG-TTCAAAGACGAACTCTGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCAGAGTTCGTCTTTGAACTCGAGTTCAAAGACGAACTCTGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCAGAGTTCGTCTTTGAACTCGAGTTCAAAGACGAACTCTGGAGC-3'

Other clones with same target sequence:

(none)