Construct: shRNA TRCN0000095959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194070.1-2801s1c1
DNA Barcode:
CCCTTAATGAATTGATGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mef2a (17258)
Gene Description:
myocyte enhancer factor 2A
Transcript:
RefSeq NM_194070.1 (NON-CURRENT)
Match location:
Position 2801 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTAATGAATTGATGACTA
Hairpin Sequence:
5'-CCGG-CCCTTAATGAATTGATGACTA-CTCGAG-TAGTCATCAATTCATTAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTAATGAATTGATGACTACTCGAGTAGTCATCAATTCATTAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTAATGAATTGATGACTACTCGAGTAGTCATCAATTCATTAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000333988