Construct: shRNA TRCN0000095980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175088.2-680s1c1
DNA Barcode:
CCGTGGAGAATCACAAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mdfic (16543)
Gene Description:
MyoD family inhibitor domain containing
Transcript:
RefSeq NM_175088.2 (NON-CURRENT)
Match location:
Position 680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGGAGAATCACAAGATAT
Hairpin Sequence:
5'-CCGG-CCGTGGAGAATCACAAGATAT-CTCGAG-ATATCTTGTGATTCTCCACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGGAGAATCACAAGATATCTCGAGATATCTTGTGATTCTCCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGGAGAATCACAAGATATCTCGAGATATCTTGTGATTCTCCACGG-3'

Other clones with same target sequence:

(none)