Construct: shRNA TRCN0000095981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175088.2-420s1c1
DNA Barcode:
CGAAGCATGTAATGAGGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mdfic (16543)
Gene Description:
MyoD family inhibitor domain containing
Transcript:
RefSeq NM_175088.2 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000095981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGCATGTAATGAGGACAA
Hairpin Sequence:
5'-CCGG-CGAAGCATGTAATGAGGACAA-CTCGAG-TTGTCCTCATTACATGCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGCATGTAATGAGGACAACTCGAGTTGTCCTCATTACATGCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGCATGTAATGAGGACAACTCGAGTTGTCCTCATTACATGCTTCG-3'

Other clones with same target sequence:

(none)